Supplements to Combat Biofilm: Part 4

Supplements to Combat Biofilm: Part 4

A majority of our antibiotics are produced from chemicals that microorganisms produce to reduce other organisms rate of growth or outright eliminate them. We use these chemicals (antibiotics) to reduce the growth of opportunistic organisms in the hope that we can reduce or eliminate sepsis.

Sometimes antibiotics are needed depending on the severity of the infection and rather it is systemic or not. Do not get me wrong, here at Fix Your Gut we definitely believe antibiotics are overused, inappropriately used, and understand the issues that stem from both. They are however a necessary component of integrative medicine. You have to weigh the pros and the cons when it comes to using anything, natural or otherwise.

This leads us to today’s supplement cis-2-decenoic acid, a potent biofilm disruptor that is a produced by an occasional opportunistic gram-negative bacterium Pseudomonas aeruginosa. 1

What is the Relationship Between Cis-2-decenoic acid and Pseudomonas aeruginosa?

Pseudomonas aeruginosa is a gram-negative (endotoxins) bacteria that is found in nature and on human skin. It is naturally part of our skin’s microbiome. It is known to cause infections, intestinal overgrowth, form biofilms, and cause excessive endotoxin inflammation when opportunistic (sepsis.) 2

When it becomes opportunistic it can cause: 3

  • Pneumonia
  • Septic shock
  • UTI
  • Intestinal overgrowth
  • Necrotising enterocolitis
  • Skin infections

You might be asking right about now, John why would I want to have anything to do with a substance that Pseudomonas aeruginosa produces since it seems like a strong opportunistic bacteria? There is a reason the bacteria when it turns opportunistic it can disrupt natural flora greatly and become dominant. One of those reasons might be its production of cis-2-decenoic acid.

Pros and Cons of Cis-2-decenoic acid supplementation

Cis-2-decenoic acid is a fatty acid produced by Psudomonas aeruginosa.


  • Inhibits S. aureus growth and biofilm formation. 4 5
  • Reduces and breaks down biofilm formation of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Streptococcus pyogenes, and Bacillus subtilis. 6
  • Reduces Candida albicans growth. 7
  • Might be useful in reducing S. mutans growth and improving oral health when brushed or swished in the mouth. 8


  • All studies done with cis-2-decenoic acid are in-vitro. We do not know if the same benefits can occur if you ingest the fatty acid, but it may correlate to topical use and biofilm reduction.
  • It may be possible that the ingestion or use of cis-2-decenoic acid may increase Pseudomonas aeruginosa survival or lead to an overgrowth. If you have a known overgrowth of the bacteria, I recommend not to use it.

What is a Natural Supplement that Contains Cis-2-decenoic acid?

A natural supplement containing cis-2-decenoic acid  has not been developed yet.

For awhile it was reported that Royal Jelly contained it, even I reported that it did. Research updates all the time and I apologize for the misinformation.

Royal Jelly contains 10-Hydroxy-2-decenoic acid, not cis-2-decenoic acid. 9

More studies need to be done with cis-2-decenoic acid and see if its positive results can be replicated in-vivo and if it can be produced and stabilized to be given in the supplemental form.

So for now, we can only theorize on the potential of using cis-2-decenoic acid to help improve our microbiome.

  1. I’ve been reading more in your biofilm series and also on other sites including nih. I’m trying to find alternative treatments for a uti – Psudomonas aeruginosa. I’m allergic to ciproflaxin. Cipro and levaquin were the only oral meds identified as effective for this. I’ve read in your work and in the nih research that lactoferrin can disrupt the biofilm. I have started that as well as taking raw garlic with each meal. Does this appear to be a safe and effective alternative?

  2. It is amazing how so many people go through life using technologies that have been created by the compilation of genius throughout history, and most of us barely understand any of it. The first paragraph of this blog post is an example of that. Very interesting stuff!

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